MOESM1 of Biological conversion of methane to putrescine using genome-scale model-guided metabolic engineering of a methanotrophic bacterium Methylomicrobium alcaliphilum 20Z

Additional file 1: Table S1. Knockout strategies suggested by OptGene algorithm for the production of putrescine. Figure S1. Knockout of speE1 check by PCR using primer outside of the flanking region. Lane 1 is the mutant strain and Lane 2 is the wild-type strain. Figure S2. The insertion of pTAC-argDJ into the genome of the engineered strain 20ZE4A. Lane 1 is the 1 kbp marker Enzynomics, Lane 2 is the wild-type strain, and Lane 3 is the engineered strain.