12935_2018_706_MOESM1_ESM.docx (1.05 MB)
MOESM1 of Arenobufagin induces MCF-7 cell apoptosis by promoting JNK-mediated multisite phosphorylation of Yes-associated protein
journal contribution
posted on 2018-12-18, 05:00 authored by Li-Juan Deng, Ming Qi, Qun-Long Peng, Min-Feng Chen, Qi Qi, Jia-Yan Zhang, Nan Yao, Mao-Hua Huang, Xiao-Bo Li, Yin-Hui Peng, Jun-Shan Liu, Deng-Rui Fu, Jia-Xu Chen, Wen-Cai Ye, Dong-Mei ZhangAdditional file 1: Table S1. The sequence-specific oligonucleotide primers. Table S2. The target siRNA duplexes. Figure S1. Representative data from the Annexin V/PI apoptosis assay. Following ABF treatment, MCF-7 cells were subjected to Annexin V/PI staining and analyzed by flow cytometry, nâ=â3. Figure S2. Distribution of YAP in the nuclei and cytoplasm of ABF-treated MCF-7 cells. Nuclear and cytoplasmic fractions were collected and evaluated by Western blotting. Lamin B1 was used as the nuclear protein control, while GAPDH was used as the cytoplasmic protein control.