MOESM1 of Arenobufagin induces MCF-7 cell apoptosis by promoting JNK-mediated multisite phosphorylation of Yes-associated protein

Additional file 1: Table S1. The sequence-specific oligonucleotide primers. Table S2. The target siRNA duplexes. Figure S1. Representative data from the Annexin V/PI apoptosis assay. Following ABF treatment, MCF-7 cells were subjected to Annexin V/PI staining and analyzed by flow cytometry, n = 3. Figure S2. Distribution of YAP in the nuclei and cytoplasm of ABF-treated MCF-7 cells. Nuclear and cytoplasmic fractions were collected and evaluated by Western blotting. Lamin B1 was used as the nuclear protein control, while GAPDH was used as the cytoplasmic protein control.