MOESM1 of Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion

Additional file 1: Figure S1. Antioxidants inhibit ADSC cell senescence during long-term in vitro expansion. After treatment with 10 μM GSH or melatonin, the ADSCs cultured for passage 3 (P3), passage 6 (P6) and passage 9 (P9) were used in the following analysis. (a) Confocal images of SPiDER-βGal staining in ADSCs (×400 magnification; scale bar, 50 μm). (b) Quantification of SPiDER-βGal fluorescence intensity (n = 3 per group; **p < 0.01). ADSCs adipose tissue-derived stem cells, GSH reduced glutathione. (TIF 1019 kb)