MOESM1 of A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virus

Additional file 1: Table S1. Primer pairs in the study. Table S2. The sensorgrams data of SPR (binding affinities of PEDV N protein to Nb2). Fig. S1. Titer of anti-N antibody in the immune camel serum detected by ELISA. Fig. S2. Construction and identification of phage library. Fig. S3. Specificity and binding activity of three nanobodies against PEDV N by ELISA. Fig. S4. Affinity analysis of PEDV N protein binding to Nbs with SPR. Fig. S5. Screening of bELISA conditions, including optimization of the concentration of PEDV N protein and the corresponding BiNb2 by a checkboard titration, sera dilution, serum incubation time, BiNb2 incubation time and incubation time of streptavidin-HRP on the performance of bELISA. Fig. S6. Specificity analysis of the bELISA by different antigens.