MOESM1 of A mechanistic investigation exploring the differential transfection efficiencies between the easy-to-transfect SK-BR3 and difficult-to-transfect CT26 cell lines

Additional file 1: Figure S1. Subcellular Trafficking of Cy5-labeled PEI/DNA Complexes in SK-BR3 Cells. (A) The intracellular trafficking of Cy5-labeled GFP reporter gene plasmid DNA (shown in red) was observed in SK-BR3 cells 1-hour, 4-hours, 24-hours, and 48-hours post-transfection via confocal microscopy. Prior to imaging, acidic organelles were stained with Lysotracker (shown in yellow) and nuclei were stained with DAPI (shown in blue). Column (A) depicts 60× magnification and Column (B) depicts Nyquist zoom of the corresponding images in column (A). Column (C) exhibits overlays of the fluorescent channels with the transmission channel for the corresponding images in column (B). Scale bar is (A) 20 μm and (B), (C) 10 μm.