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MOESM15 of Co-expression network analysis predicts a key role of microRNAs in the adaptation of the porcine skeletal muscle to nutrient supply

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posted on 2020-01-17, 04:52 authored by Emilio Mármol-Sánchez, Yuliaxis Ramayo-Caldas, Raquel Quintanilla, Tainã Cardoso, Rayner González-Prendes, Joan Tibau, Marcel Amills
Additional file 15: Figure S1. Sequencing depth obtained for samples analyzed in each one of the two contrasts (AL-T0/AL-T1 and AL-T0/AL-T2). Figure S2. Joint Principal Component Analysis (PCA) clustering of gluteus medius skeletal muscle samples (11 AL-T0, 12 AL-T1 and 12 AL-T2 samples) according to the expression profiles of (A) mRNAs, (B) microRNAs and (C) lincRNAs. Figure S3. Phylogenetically conserved 7mer-8 m predicted binding sites in the 3′- UTR of the pig PDK4 gene for (A) ssc-miR-148a-3p and (B) ssc-miR-493-5p porcine miRNAs. The TargetScan software was used for generating conservation graphs across the investigated mammalian species. Red nucleotides show complementary matching base-pairs between the seed of the mature miRNA and the 3’UTR of the pig PDK4 gene. Figure S4. Gene co-expression module association with meat quality and fatty acids composition traits in the AL-T0/AL-T1 contrast as determined with the WGCNA tool. Figure S5. Gene co-expression module association with meat quality and fatty acids composition traits in the AL-T0/AL-T2 contrast as determined with the WGCNA tool.

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Ministerio de Economía y Competitividad

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