MOESM12 of Ephemeral-habitat colonization and neotropical species richness of Caenorhabditis nematodes

Additional file 12. Crosses of novel species (C. dolens, C. astrocarya) with known Caenorhabditis species [3]. Reciprocal crosses between different species and strains were performed as previously described [3], allowing for mating between 5–10 males and 5–10 females on a 55 mm diameter NGM plate. (A) Results of reciprocal crosses between the novel species C. dolens and presumptively close known relatives C. angaria, C. castelli, C. sp. 8, and C. astrocarya (B) Results of crosses between the novel species C. astrocarya and close known relatives, C. guadeloupensis and C. dolens.