13072_2016_64_MOESM10_ESM.eps (922.14 kB)
MOESM10 of Setdb1-mediated H3K9 methylation is enriched on the inactive X and plays a role in its epigenetic silencing
figure
posted on 2016-05-18, 05:00 authored by Andrew Keniry, Linden Gearing, Natasha Jansz, Joy Liu, Aliaksei Holik, Peter Hickey, Sarah Kinkel, Darcy Moore, Kelsey Breslin, Kelan Chen, Ruijie Liu, Catherine Phillips, Miha Pakusch, Christine Biben, Julie Sheridan, Benjamin Kile, Catherine Carmichael, Matthew Ritchie, Douglas Hilton, Marnie BlewittAdditional file 10. DNA methylation is largely unaltered upon Setdb1 depletion outside of X-linked CpG islandseRRBS data from Setdb1 +/+ Xist ∆A/+ 129/CAST transduced with Nons (Female) or Setdb1 +/gt Xist ∆A/+ 129/CAST transduced with shSetdb1.6 (Setdb1 depleted) MEFs, showing mC for individual CpGs on the Xa and Xi in Female (n = 3) and Setdb1 depleted female samples (n = 2), for CpGs outside CGIs on the X chromosome (Student’s two-tailed t-test) as log2(mC/C) (a), within CGIs on autosomes (b), outside CGIs on autosomes (c) and within CGIs with greater than 20% mC on autosomes (d). Note that autosomal data used is not allele specific. Dotted line indicates median for Xi in Female.
