13046_2019_1505_MOESM10_ESM.pdf (10.19 MB)
MOESM10 of A pre-existing population of ZEB2+ quiescent cells with stemness and mesenchymal features dictate chemoresistance in colorectal cancer
journal contribution
posted on 2020-01-08, 04:55 authored by Federica Francescangeli, Paola Contavalli, Maria Laura De Angelis, Silvia Careccia, Michele Signore, Tobias Longin Haas, Federico Salaris, Marta Baiocchi, Alessandra Boe, Alessandro Giuliani, Olga Tcheremenskaia, Alfredo Pagliuca, Ombretta Guardiola, Gabriella Minchiotti, Lidia Colace, Antonio Ciardi, Vito D’Andrea, Filippo La Torre, JanPaul Medema, Ruggero De Maria, Ann ZeunerAdditional file 10: Figure S4. In vivo effects of ZEB2 overexpression in CCSCs. a Volume of xenografts derived from CCSCs transduced with pLenti-GFP (Vector, black line/triangles) or with pLenti-GFP-ZEB2 (ZEB2, red line/squares). Graph shows the mean ± SEM, 6 tumors/group. **P < 0.01 from two-tailed t test. b Left panels: representative confocal images of xenograft sections derived from tumors obtained with primary cells transduced with pLenti-GFP (Vector) and GFP-ZEB2 (ZEB2). Sections were stained with anti-Ki67 (red). Scale bar, 70 μm. Quantification (right panel) was performed on 5 fields /group. *P < 0.05 from two-tailed t test. AU, arbitrary units. c Cell cycle analysis of GFP+ cells FACS-isolated from Vector and ZEB2-transduced tumors obtained with primary cells. d qRT-PCR analysis of the indicated transcripts in Vector- and ZEB2-transduced tumors obtained with primary cells, n = 3. *P < 0.05 and **P < 0.01 from two-tailed t test. n.s. = not significant by t test. Values are the mean ± SD.
