This file includes 2177 DICOM files of a male Squatina squatina specimen housed in the spirit collection of Naturalis Biodiversity Center, Leiden, the Netherlands with registrationnumber RMNH.PISC.24047. The specimen is scanned in a medical CT scanner (Toshiba Aquilion 64) at the Leiden University Medical Center, the Netherlands at 100 kV and 150 mAs with a slice thickness of 0.5 mm.