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bioreactor_MCF7_HDF_alginate_docetaxel_day-20_sample-6.ome.tif (94.36 MB)

Example fluorescent 3D image stack from bioreactor breast cancer co-culture treated with docetaxel

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posted on 2017-11-20, 10:43 authored by Emma Jane Davies, Meng Dong, Katja Maria Närhi, Sami Blom, Catarina Brito, ralph graeser, Suzana Vidic, Annika Osswald, Erwin Boghaert, Ronald De Hoogt, Marta F Estrada, Michael Barbier, Vítor Espirito Santo, Kjersti Gjerde, Hanneke J.A.A. van Zoggel, Yolanda Chong
Example confocal image stack from MCF7 (GFP) 3D co-culture (Human Dermal Fibroblasts) embedded in alginate capsules and grown in a stirred tank bioreactor. The culture is treated with docetaxel. The image is a 16-bit multi-channel image stack containing the RFP, GFP, and EdU channel. The pixelsize is ( 0.7 x 0.7 x 1 ) micron (20x magnification image). The image is in (OME.)TIF-format, where OME.TIF-images contain metadata which can be extracted by e.g. the Bioformats ImageJ plugin.

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