Data for De novo Comparative Transcriptome Analysis of Genes Differentially Expressed in the Scion of Homografted and Heterografted Tomato Seedlings
2020-01-21T10:56:20Z (GMT) by
This data record contains a single .xml file holding annotation data, analysis results and primer sequences for comparitive transcriptome analysis of homografted and heterografted tomato seedlings. The data tables correspond to supplementary tables 1-4 in the related research publication.
The data file can be accessed via MS Excel, or openly available spreadsheet or notepad software.
The related study investigates potential molecular mechanisms involved in plant grafting using genetic approaches, in particular underlying mechanisms implicated in the regulation of development and stress responses at the whole-plant level. The investigation employs transcriptome analysis of grafted plants to reveal specific genes involved in the regulation of grafting-induced physiological responses. Transcriptome results are validated using quantitative real-time PCR (qPCR) analysis.
The study provides a foundation for the elucidation of the differentially expressed genes (DEGs) in the scion of hetero- and homografted seedlings. Grafting is significant in vegetable production to enhance plant vigour through the avoidance of soil pathogens and the increased tolerance to low temperature, high salinity, and drought.
The data files contains the following elements:
Supplementary Table S1. The DEGs and functional annotation detected between hetero- and homografted tomato seedlings. Including: gene names, fold changes, biotypes and other annotations.
Supplementary Table S2. KEGG pathway enrichment analysis detected between hetero- and homografted tomato seedlings. Including: athway name, mapping and gene counts.
Supplementary Table S3. Familial classification of transcription factors differentially expressed in the scion of heterografted tomato seedlings, Including: Gene name, References sepcies, family and reference gene introduction.
Supplementary table S4. Quantitative real-time PCR (RT-qPCR) validated primer sequences for selected differentially expressed genes detected between hetero- and homografted tomato seedlings.
Raw FASTQ files were deposited in the NCBI Sequence Read Archive under the accession number SUB5937299