Additional file 9: of New perspectives on the plant PARP family: Arabidopsis PARP3 is inactive, and PARP1 exhibits predominant poly (ADP-ribose) polymerase activity in response to DNA damage

Figure S8 AtPARP1 was responsible for the generation of most of the PAR signals under bleomycin and zeocin treatments. (A) PAR signals in different parp1 and parp2 mutants after mock (H2O) treatment for 24 h and 48 h, respectively. (B) PAR signals in different parp1 and parp2 mutants after 200 μg/mL zeocin or 25 μg/mL bleomycin treatment for 24 h. (C) PAR signals in different parp1 and parp2 mutants after 200 μg/mL zeocin or 25 μg/mL bleomycin treatment for 48 h. 10-d-old seedlings were treated with zeocin or bleomycin for different time periods and then the total proteins were extracted and detected using anti-pan-ADPR reagent. Tubulin was detected using an anti-tubulin antibody to indicate the protein loading amounts. TUB, tubulin; @-pan-ADPR, anti-pan-ADPR reagent. (PDF 9141 kb)