Additional file 9 of Influenza vaccination in early Alzheimer’s disease rescues amyloidosis and ameliorates cognitive deficits in APP/PS1 mice by inhibiting regulatory T cells

Additional file 9: Figure S9. Augmented peripheral immunosuppression reversed the immunoreactivity of GFAP reduced by IIV in APP/PS1 mice. (A-D) Representative microscopy images of GFAP (red) and Hoechst nuclear staining (blue) (scale bar, 200 μm) in the AD (A), AD+IIV (B), IIV+ATRA (C) and IIV+Vehicle (D) mice. (E) Quantification of GFAP immunostaining in the hippocampus in the four groups. (n > 6, one-way ANOVA and LSD post hoc analysis, *P < 0.05, **P < 0.01).