Additional file 9: of FAF1 mediates necrosis through JNK1-mediated mitochondrial dysfunction leading to retinal degeneration in the ganglion cell layer upon ischemic insult

Figure S8. JNK1 leads to cell death in GCL upon ischemic stress. (a) The mice were sacrificed 1, 6, and 24 h after induction of intraocular hypertension. Immunoblot assay showing JNK1 activation in response to retinal ischemia in retinas of Dkk3-Cre;Faf1+/+ mice (n = 3 eyes). (b) Immunoblot assay showing JNK1 activation in response to retinal ischemia in retinas of Dkk3-Cre;Faf1+/+ mice (n = 3 eyes). (c) The mice were sacrificed 1, 3, 5, and 7 day after induction of intraocular hypertension. The enucleated eyes were stained with HE. Histological images showing alterations in retinal morphology. (d) The graph shows the number of cells in the GCL of the central region of the retina in HE-stained samples (n = 5 eyes). The data (b and d) are expressed as the mean ± S.E.M. of three independent experiments. Statistical comparisons were evaluated with ANOVA followed by Tukey’s HSD (b and d) post hoc analysis. ***P < 0.001, **P < 0.01, and *P < 0.05. (PDF 117 kb)