Additional file 9: of Circular RNA circNRIP1 acts as a microRNA-149-5p sponge to promote gastric cancer progression via the AKT1/mTOR pathway

Figure S9. (A). We observed that only the wild-type plasmid (#4), and neither the I1QB nor I3QB deletion constructs (#1-3), could overexpress circNRIP1 according to northern blotting in BGC-823 cells. (B). We observed that only the wild-type plasmid (#4), and neither the I1QB nor I3QB deletion constructs (#1-3), could overexpress circNRIP1 according to qRT-PCR in BGC-823 cells. (C). We knocked down QKI and observed a significant reduction in circNRIP1 but not pre-mNRIP1 or mNRIP1 in BGC-823 cells. (D). Enrichment of I1QB and I3QB was observed when we used an antibody against QKI in BGC-823 cells. (E). The donor patients were clinically characterized. (F). The engrafted tumours were histopathologically analysed, scale bar = 100 µm. (G). We found that circNRIP knockdown in vivo significantly blocked tumour growth in terms of tumour weight and volume relative to the negative control group, whereas overexpression of circNRIP1 promoted the growth of xenografted tumours. All data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. (TIF 6448 kb)