Additional file 9: of Cathepsin L activated by mutant p53 and Egr-1 promotes ionizing radiation-induced EMT in human NSCLC

Figure S4. The endogenous mut-p53 regulates the transcription of CTSL under IR in two lung cancer cell lines. A) VMRC-LCD (p53-R175H) and H1838 (p53-R273L) cells were treated with/ without IR and harvested for ChIP assay to verify the interaction between endogenous mut-p53 and the promoter of CTSL (up panel). The corresponding quantities of CTSL promoter expression were shown (down panel). B) Cells were treated as mentioned above and harvested for ChIP assay to verify the interaction between Egr-1 and the promoter of CTSL under/ or not IR (up panel). The corresponding quantities of CTSL promoter expression were shown (down panel). C) ChIP assay was analyzed to verify the interaction between mut-p53 and the promoter of Egr-1 in two endogenous mut-p53 cell lines with or without IR treatment. The recruitment of endogenous mut-p53 to the Egr-1 promoter was shown (up panel). The corresponding quantities of Egr-1 promoter expression were shown (down panel). Data are shown as mean ± S.D., n = 3, *P < 0.05, **P < 0.01, *** P < 0.001, Student’s t-test. (JPG 632 kb)