Additional file 9: of A rare population of tumor antigen-specific CD4+CD8+ double-positive αβ T lymphocytes uniquely provide CD8-independent TCR genes for engineering therapeutic T cells

Cytokine and cytotoxic molecule production from TCR-transduced T cells. Whole PBMC, CD4+ or CD8+ T cells that were transduced with 19305DP-TCR or CD8SP-TCR gene were cocultured with A375 and the culture supernatant was harvested at day 1 - day 4. TNF-α, IL-2, granzyme B (Gzm B) and perforin levels in the culture supernatant were measured by ELISA. (PDF 100 kb)