Additional file 8: Figure S7. of Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A

(A) Western blot analysis of APP and Aβ in Swe N2a cells treated with 40 μg/ml cycloheximide (CHX) at different times in hours (h) before harvest. Cell culture media was replaced with fresh media 24 h before harvest. For quantification, values are normalized against actin and expressed as percentage of control, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (ANOVA with Dunnett’s multiple comparisons test, compared to ctr). (B) Confocal images of 6E10 and Golgi marker GM130 in Swe N2a cells treated with 40 μg/ml CHX for the depicted times. (PDF 2263 kb)