13024_2017_203_MOESM8_ESM.pdf (2.21 MB)
Additional file 8: Figure S7. of Aβ accumulation causes MVB enlargement and is modelled by dominant negative VPS4A
Version 2 2017-09-26, 15:30
Version 1 2017-08-24, 08:23
journal contribution
posted on 2017-08-23, 05:00 authored by Katarina Willén, James Edgar, Takafumi Hasegawa, Nobuyuki Tanaka, Clare Futter, Gunnar Gouras(A) Western blot analysis of APP and Aβ in Swe N2a cells treated with 40 μg/ml cycloheximide (CHX) at different times in hours (h) before harvest. Cell culture media was replaced with fresh media 24 h before harvest. For quantification, values are normalized against actin and expressed as percentage of control, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (ANOVA with Dunnett’s multiple comparisons test, compared to ctr). (B) Confocal images of 6E10 and Golgi marker GM130 in Swe N2a cells treated with 40 μg/ml CHX for the depicted times. (PDF 2263 kb)
