Additional file 8: of Echinococcus granulosus Antigen B binds to monocytes and macrophages modulating cell response to inflammation

Determination of arginase activity in bone marrow-derived macrophages stimulated with EgAgB. Arginase activity was evaluated in BMDM incubated with PBSEB (control), immunopurified EgAgB (0.2–20 μg/mL), or murine IL-4 (2.5 ng/ml) as a control of macrophage alternative activation. Arginase activity was expressed as mU per million of BMDM. The graph is representative of three independent experiments. Bars indicate SEM of analytical triplicates. Asterisks (*) indicate significant differences with respect to the control (medium) according to one-way ANOVA analysis followed by Tukey’s post-test (p < 0.05). (TIF 27 kb)