Additional file 8: of Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5′ splice site strength

PRPF8 depletion alters the dynamics of RNA splicing during transcription. a Raw data used to calculate co-transcriptional splicing ratio in Fig. 8a is shown. Normalized intron expression for first introns (fk, orange) and last introns (fI, blue) is shown for each independent depletion experiment (three for control siRNA, and four for PRPF8 siRNA). b The 5′ splice site strength of first and last introns are similar. The 5′ splice site strength was analyzed as described in “Materials and methods” for the first and last introns in the 2380 genes considered for the analysis of the co-transcriptional splicing ratio. The p value is indicated. c PRPF8 depletion alters the dynamics of RNA splicing during transcription. The kinetics of transcription and splicing recovery of the Separase gene following release from drug-induced transcriptional arrest were measured in control siRNA-treated and PRPF8-depleted cells in a similar fashion to that shown in Fig. 8d. (PDF 109 kb)