Additional file 8: of Microglia prevent peripheral immune cell invasion and promote an anti-inflammatory environment in the brain of APP-PS1 transgenic mice

Figure S8. Immunohistochemical staining for proliferating cell nuclear antigen (PCNA) to analyze the proliferative activity of CD8+ T-cells in APP-PS1 and microglia ablated APP-PS1 brains. CD8+ T-cells in the hippocampus (A) and cortex (B) of APP-PS1 mice were not observed to proliferate (arrows). Interestingly, after microglia ablation in APP-PS1 mice, no increase in proliferative activity was detected, suggesting CD8+ T-cells to rather infiltrate the brain than being locally generated. Dapi (blue) was used as nucleus stain. Scale: 50 Οm (A, B). (TIF 4664 kb)