Additional file 8: of Manipulation of spermatogonial stem cells in livestock species

Compared to cryopreservation of single cell suspension, the approach of whole testis tissue cryopreservation is more challenging; this is because tissue requires longer exposure to cryoprotectants and this can result in higher cellular toxicity before freezing [135]. However, the cryopreservation of the whole testis tissue is recognized as promising, at least in human regenerative medicine. In this approach, the SSCs purified from the thawed tissue and propagated subsequently in vitro [198]. Readers interested in this approach should refer the very recent review [199]. (DOCX 12 kb)