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Additional file 8 of MARPLE, a point-of-care, strain-level disease diagnostics and surveillance tool for complex fungal pathogens
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posted on 2019-08-13, 04:00 authored by Guru Radhakrishnan, Nicola Cook, Vanessa Bueno-Sancho, Clare Lewis, Antoine Persoons, Abel Mitiku, Matthew Heaton, Phoebe Davey, Bekele Abeyo, Yoseph Alemayehu, Ayele Badebo, Marla Barnett, Ruth Bryant, Jeron Chatelain, Xianming Chen, Suomeng Dong, Tina Henriksson, Sarah Holdgate, Annemarie Justesen, Jay Kalous, Zhensheng Kang, Szymon Laczny, Jean-Paul Legoff, Driecus Lesch, Tracy Richards, Harpinder Randhawa, Tine Thach, Meinan Wang, Mogens Hovmøller, David Hodson, Diane SaundersRNA-seq analysis of the 51 Ethiopian Pst isolates using the 242 genes defined herein shows they are genetically closely related. Phylogenetic analysis was carried out using the 51 Pst isolates collected in Ethiopia in the 2017/2018 growing season and the 301 global Pst isolates, using a maximum-likelihood model and 100 bootstraps. The 51 Ethiopian Pst isolates grouped in two closely related clades. One of the two clades also grouped closely with Pst isolates from genetic group 2, however, this was supported by a low bootstrap value that may be indicative of uncertainty in the grouping of these isolates (Additional file 12). Scale indicates the mean number of nucleotide substitutions per site. (EPS 1459 kb)
