Additional file 8: of FAF1 mediates necrosis through JNK1-mediated mitochondrial dysfunction leading to retinal degeneration in the ganglion cell layer upon ischemic insult

Figure S7. FAF1 enhances necrosis in RGC5 cells upon ischemic stress induced by oxygen glucose deprivation. (a) Left panel: RGC5 cells were transfected with the indicated concentration of Flag-FAF1 plasmid. At 48 h after transfection, the cells were untreated or treated with oxygen glucose deprivation for 8 h. Cell death was determined by measuring PI uptake using a flow cytometer (n = 3). Right panel: representative immunoblots show the Flag, FAF1 and β-actin expression levels. (b) RGC5 cells were transfected with VC or Flag-FAF1 plasmids. At 48 h after transfection, the cells were treated with oxygen glucose deprivation for the indicated times. Cell death was determined by flow cytometry (n = 3). The data (a and b) are expressed as the mean ± S.E.M. of three independent experiments. Statistical comparisons were evaluated with ANOVA followed by Tukey’s HSD (a and b) post hoc analysis. ***P < 0.001, and **P < 0.01. (PDF 65 kb)