Additional file 8: of Cathepsin L activated by mutant p53 and Egr-1 promotes ionizing radiation-induced EMT in human NSCLC

Figure S3. The expression of CTSL significantly increased under IR in H1299 cells with the R175H and R273H mutation. Western blotting analysis of p53 and CTSL in H1299 cells transfected with Flag, Flag-p53, Flag-p53 (R175H), Flag-p53 (R248Q) and Flag-p53 (R273H) plasmid respectively (up panel). Band intensity of active-CTSL was quantified and relative-fold change under IR vs. without IR is presented (down panel). Data were shown as mean ± S.D., n = 3, *, P < 0.05, ***, P < 0.001, Student’s t-test. (JPG 158 kb)