Additional file 8: Figure S7. of MicroRNA-195 acts as an anti-proliferative miRNA in human melanoma cells by targeting Prohibitin 1

PHB1 protects UACC-62 melanoma cells of chemotherapy induced cell-death. UACC-62 melanoma cells stably expressing either pcDNA3.1-EV or ORF-PHB1 were treated with cisplatin (cis; 5 or 10 μM) or temozolomide (tmz; 50 or 250 μM) for 48 h. The percentage of viable cells (a) and annexin V positive/PI negative cells (b) were determined using Annexin V Conjugates for Apoptosis Detection kit for flow cytometry (Life Technologies). Statistical analysis was carried out using Two-Way ANOVA followed by Bonferroni post-test and are reported by mean ± SD. Representative data of three independent experiments are reported. ***P ≤ 0.001. (JPEG 62 kb)