Additional file 8: Figure S7. of LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors

Sustained YAP1 expression hinders smooth muscle cell differentiation. (A) Serial transverse sections of E7 control GFP- and YAP1-expressing stomachs analysed either by in situ hybridization using the ENV riboprobe or by immunofluorescence with anti-αSMA and anti-CALPONIN antibodies. Nuclei were visualized with Hoechst. (B) Western blot analysis of YAP and phospho-YAP (72 kDa) levels in protein extracts from control GFP- and YAP1-expressing stomachs. Equal loading was verified by GAPDH expression (37 kDa). Graph represents the quantification of western blot data. Normalized expression levels were converted to fold changes. Values are presented as the mean ± standard deviation of n = 6 controls vs. n = 6 YAP1-expressing stomachs. *P < 0.05 by one-tailed Mann–Whitney test. (C) RT-qPCR analysis of relative mRNA expression in E7 control GFP- and YAP1-expressing stomachs. Data were normalized to GAPDH expression. Normalized expression levels were converted to fold changes. Values are presented as the mean ± standard deviation of n = 8 controls vs. n = 6 YAP1-expressing stomachs. *P < 0.05 by one-tailed Mann–Whitney test. (JPG 2004 kb)