12862_2016_790_MOESM8_ESM.ai (293.08 kB)

Additional file 8: Figure S6. of Shedding light on the expansion and diversification of the Cdc48 protein family during the rise of the eukaryotic cell

Download (293.08 kB)
posted on 18.10.2016 by Nickias Kienle, Tobias Kloepper, Dirk Fasshauer
Evolutionary tree of the canonical N-domains of different Cdc48 family members. Our analysis revealed that some Cdc48 family members have conserved N-domains. These N-domains are composed of two subdomains Na and Nb. Na is a double-ψβ barrel fold; the Nb domain is an αβ roll. The tree was constructed using the well-conserved homologous N-domains of Cdc48, N-ethylmaleimide-sensitive factor (NSF), Pex1 (N1), Pex6 (N2), Spaf, Spaf-like, and archaeal VCP-like ATPase of Thermoplasma acidophilum (VAT). We did not include the very divergent second N-domains of Pex1 (N2) and Pex2 (N1, see Fig. 1). Note that the N-domains of NSF and of Pex1 (N1) are more divergent than the N domains of the other family members. The different family members are highlighted using the same colors as in Figs. 2 and 4. (AI 293 kb)


Deutsche Forschungsgemeinschaft