Additional file 8: Figure S6. of Shedding light on the expansion and diversification of the Cdc48 protein family during the rise of the eukaryotic cell

Evolutionary tree of the canonical N-domains of different Cdc48 family members. Our analysis revealed that some Cdc48 family members have conserved N-domains. These N-domains are composed of two subdomains Na and Nb. Na is a double-ψβ barrel fold; the Nb domain is an αβ roll. The tree was constructed using the well-conserved homologous N-domains of Cdc48, N-ethylmaleimide-sensitive factor (NSF), Pex1 (N1), Pex6 (N2), Spaf, Spaf-like, and archaeal VCP-like ATPase of Thermoplasma acidophilum (VAT). We did not include the very divergent second N-domains of Pex1 (N2) and Pex2 (N1, see Fig. 1). Note that the N-domains of NSF and of Pex1 (N1) are more divergent than the N domains of the other family members. The different family members are highlighted using the same colors as in Figs. 2 and 4. (AI 293 kb)