Additional file 8: Figure S5. of miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1

Effect of c-Myc on TIA1 mRNA level and the efficacies of TIA1 siRNA and overexpression vector. (A) Quantitative RT-PCR analysis of TIA1 mRNA levels in SW480 cells transfected with control siRNA, si-c-Myc, control plasmid or c-Myc plasmid. (B and C) Western blot analysis of TIA1 protein levels in SW480 cells transfected with control siRNA, si-TIA1, control vector or TIA1 vector. B: representative images; C: quantitative analysis. (D) Quantitative RT-PCR analysis of TIA1 mRNA levels in SW480 cells transfected with control siRNA, si-TIA1, control vector or TIA1 vector. ***P < 0.001. (TIF 602 kb)