Additional file 8: Figure S3. of Sex chromosomes drive gene expression and regulatory dimorphisms in mouse embryonic stem cells

Allele-specific expression analysis for imprinted gene Cdkn1c. RT-PCR was followed by allele-specific restriction digest of the Cdkn1c coding sequence and polyacrylamide gel analysis. A single nucleotide polymorphism in the M. castaneus allele generates a restriction site for aTaqI. Two different F1 hybrid ES cell lines each derived from reciprocal crosses of C57BL/6 (B) and CAST/EIJ (C) mice exhibited monoallelic (BxC) and biased expression (CxB) from the maternal allele. The first lane is the marker, the last two lanes are digested controls from PCR products from B and C genomic DNA.