Additional file 7: of c-MYC overexpression induces choroid plexus papillomas through a T-cell mediated inflammatory mechanism

Figure S5. Inflammatory cells in c-MYC overexpressing human CPT. (A) CD8 (cytotoxic T-lymphocyte, a subtype of T-cells) immunohistochemistry shows no significant difference in infiltrate cell counts between c-MYC+ and c-MYC-tumours; quantification shown in bar graph on right hand side; Mean ± SEM; n = 10 for c-MYC+ and n = 9 for c-MYC- cohorts; ns – no statistical significance. (B) CD20 (B-lymphocyte marker) immunohistochemistry shows no significant difference in infiltrate cell counts between c-MYC+ and c-MYC- tumours; quantification shown in bar graph on right; Mean ± SEM; n = 11 for c-MYC+ and n = 9 for c-MYC- cohorts; ns – no statistical significance. (C, D) CD3 and F4-80 immunohistochemistry in NestinCre;c-MycSTOPFlox mice at different ages revealed no statistically significant increase in the number of T-lymphocyte (C) or macrophage (D) in the CP as compared to wild-type mice until tumour development at 9 months of age. Quantification bar graph on right side of C & D, Mean ± SEM; n for CD3 and F4-80 studies in time points P15 = 4, 3m = 3, 6m = 6, 9m = 4 for both mCP and mCPT cohorts; * P<0.05. Scale bar = 125 μm (A, B, C, D). (JPG 1187 kb)