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Additional file 7: of c-MYC overexpression induces choroid plexus papillomas through a T-cell mediated inflammatory mechanism

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posted on 2019-05-29, 05:00 authored by Ashirwad Merve, Xinyu Zhang, Nicola Pomella, Serena Acquati, Joerg Hoeck, Anaelle Dumas, Gabriel Rosser, Yichen Li, Jennie Jeyapalan, Silvia Vicenzi, Qianhai Fan, Zeng Yang, Arianna Sabò, Denise Sheer, Axel Behrens, Silvia Marino
Figure S5. Inflammatory cells in c-MYC overexpressing human CPT. (A) CD8 (cytotoxic T-lymphocyte, a subtype of T-cells) immunohistochemistry shows no significant difference in infiltrate cell counts between c-MYC+ and c-MYC-tumours; quantification shown in bar graph on right hand side; Mean ± SEM; n = 10 for c-MYC+ and n = 9 for c-MYC- cohorts; ns – no statistical significance. (B) CD20 (B-lymphocyte marker) immunohistochemistry shows no significant difference in infiltrate cell counts between c-MYC+ and c-MYC- tumours; quantification shown in bar graph on right; Mean ± SEM; n = 11 for c-MYC+ and n = 9 for c-MYC- cohorts; ns – no statistical significance. (C, D) CD3 and F4-80 immunohistochemistry in NestinCre;c-MycSTOPFlox mice at different ages revealed no statistically significant increase in the number of T-lymphocyte (C) or macrophage (D) in the CP as compared to wild-type mice until tumour development at 9 months of age. Quantification bar graph on right side of C & D, Mean ± SEM; n for CD3 and F4-80 studies in time points P15 = 4, 3m = 3, 6m = 6, 9m = 4 for both mCP and mCPT cohorts; * P<0.05. Scale bar = 125 μm (A, B, C, D). (JPG 1187 kb)

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