Additional file 7: of WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1

Figure S1. Expression of WTAP in cell lines and functional investigations of WTAP. a Expression and survival analysis of WTAP in HCC (data from TCGA, analyzed with UALACN, http://ualcan.path.uab.edu/analysis.html ); b, c mRNA (b) and protein (c) level of WTAP in an immortalized hepatic cell line (QSG-7701) and nine HCC cell lines; d, e Negative control vector or Flag-WTAP was transfected into HCCLM3 (d) or SMMC-7721 (e) with the overexpression efficiency determined. Proliferation capacities were detected by CCK-8, colony formation assay; f, g Representative images and bar charts of cell migration and invasion ability in Huh7 cells with WTAP knockdown (siRNA or shRNA) or negative control detected by transwell and matrigel transwell assays (scale bar, 100 μm). (TIF 3566 kb)