Additional file 7: of UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1

Figure S4. UBL4A-induced Autophagy inhibition is related with LAMP1 rather than ATG5 or ATG7. a The expression of ATG5 and ATG7 in CFPAC-1 and BxPC-3 of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A) were analyzed by western blotting. b qRT-PCR assays examined the expression of both UBL4A and LC3B mRNA in each of 69 PDAC tissues, and the relevance was listed in each blot (r2 = 0.2879, P < 0.0001). c-d qRT-PCR analyses of LAMP1 in four PDAC cell lines of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A). e The expressions of LAMP1 and UBL4A in four PDAC cell lines of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A) were calculated. The statistical significance between different groups was calculated with Student t-test. Data are shown as the mean ± SD of three replicates; *P < 0.05, **P < 0.01; ***P < 0.001; ns: not significant. (PDF 279 kb)