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Additional file 7: of UBL4A inhibits autophagy-mediated proliferation and metastasis of pancreatic ductal adenocarcinoma via targeting LAMP1

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posted on 09.07.2019 by Hongze Chen, Le Li, Jisheng Hu, Zhongjie Zhao, Liang Ji, Chundong Cheng, Guangquan Zhang, Tao zhang, Yilong Li, Hua Chen, Shangha Pan, Bei Sun
Figure S4. UBL4A-induced Autophagy inhibition is related with LAMP1 rather than ATG5 or ATG7. a The expression of ATG5 and ATG7 in CFPAC-1 and BxPC-3 of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A) were analyzed by western blotting. b qRT-PCR assays examined the expression of both UBL4A and LC3B mRNA in each of 69 PDAC tissues, and the relevance was listed in each blot (r2 = 0.2879, P < 0.0001). c-d qRT-PCR analyses of LAMP1 in four PDAC cell lines of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A). e The expressions of LAMP1 and UBL4A in four PDAC cell lines of different groups (Vector, LV-UBL4A-Flag, shCtrl, LV-shUBL4A) were calculated. The statistical significance between different groups was calculated with Student t-test. Data are shown as the mean ± SD of three replicates; *P < 0.05, **P < 0.01; ***P < 0.001; ns: not significant. (PDF 279 kb)

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