Additional file 7: of Star-related lipid transfer protein 10 (STARD10): a novel key player in alcohol-induced breast cancer progression

Figure S5. STARD10 silencing increases cell malignancy in vitro. MCF-7 and SKBR-3 cells were transfected with two different STARD10 siRNAs (10 nM) for 48 h. (A)(D) Efficiency of STARD10 silencing was determined by qRT-PCR. Data are expressed as (mean ± SE) from 3 independent experiments performed in triplicates.*p < 0.001 vs. Sc MCF-7 cells; *p < 0.001 vs. Sc SKBR-3 cells (B)(E) MTT assay showing viability of MCF-7 cells transfected with two different StarD10 silencers. Data are expressed as (mean ± SE) from 3 independent experiments performed in triplicates. *p < 0.04 vs. Sc MCF-7 cells; *p < 0.03 vs. Sc SKBR-3 cells. (C)(F) Graphs of migration assay. Data are expressed as (mean ± SE) from 4 independent experiments performed in triplicates. 48 h. Results are shown as percent of wound with compared to Sc at 0 h and 48 h. Data are expressed as (mean ± SE) from 3 independent experiments performed in triplicates. *p < 0.04 vs. Sc 0 h MCF-7 cells; †p < 0.05 vs. Sc 48 h MCF-7 cells. *p < 0.05 vs. Sc 0 h; †P < 0.05 vs. Sc 48 h SKBR-3 cells. (PPTX 112 kb)