Additional file 7: of Protein arginine methyltransferase 3-induced metabolic reprogramming is a vulnerable target of pancreatic cancer

Figure S6. Advanced severe immunodeficiency (ASID) mice were housed under standard conditions. pLK0.1- and sh-PRMT3-overexpressing Miapaca-2 cells (1 × 107) were suspended in 50 μl PBS mixed with 30 μl Matrige and subcutaneously injected into the left flank of the mice. Tumor burden was monitored with digital calipers twice per week. Two weeks after injection, tumors were harvested and tumor weight was measured. Apoptosis of tumor tissues was analyzed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. The percentage of cell death was determined by counting the number of TUNEL-positive cells in three independent fields of different slides using ImageJ software. The results showed that PRMT3 knockout suppressed tumor growth and increased cell apoptosis. Data represented mean ± SEM. Obtained from 5 mice in each group. (PDF 1917 kb)