Additional file 7: of Microglia affect α-synuclein cell-to-cell transfer in a mouse model of Parkinson’s disease

Figure S7. Increases in pro-inflammatory signaling following LPS and IL-4 striatal injections. a Western blots of striatal tissue used to assess mannose receptor levels and NF-κB signaling in LPS- and IL-4-injected mice, normalized to β-actin. Pon S for loading reference. Quantification of contralateral and ipsilateral Str signals is shown below. * = p < 0.05; error bars represent S.E.M. b Confocal 3D reconstructions of mannose receptor signal (red) within microglia (Iba-1, green) from grafted control, LPS-, and IL-4-injected mice. Nuclei are stained blue and huα-syn is white; scale bars = 5 μm. c Summary of the LPS and IL-4 inflammatory profiles of injected mice. Pon S; Ponceau S, Str; striatum. (TIF 124784 kb)