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Additional file 7: of Inhibition of mitochondrial respiration prevents BRAF-mutant melanoma brain metastasis

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posted on 2019-04-10, 05:00 authored by Terje Sundstrøm, Lars Prestegarden, Francisco Azuaje, Synnøve Aasen, Gro Røsland, Jobin Varughese, Marzieh Bahador, Simon Bernatz, Yannick Braun, Patrick Harter, Kai Skaftnesmo, Elizabeth Ingham, Lisa Mahakian, Sarah Tam, Clifford Tepper, Kjell Petersen, Katherine Ferrara, Karl Tronstad, Morten Lund-Johansen, Rudi Beschorner, Rolf Bjerkvig, Frits Thorsen
Figure S6. β-sitosterol treatment effect is not model specific. a Experiment overview. Mice were injected intracardially with 5 × 105 PC14_PE6_Br2 cells. Test groups received daily i.p. injections of 0.1 mL vehicle (olive oil; n = 4) or β-sitosterol 5 mg/kg (n = 6). Treatment started one week prior to tumor cell injections and was continued until euthanization. b Body weight from week zero to four for vehicle and β-sitosterol-treated mice. c Number and d volume of brain metastases at MRI at three weeks (T1-weighted with contrast). Total volume of brain metastases per animal compared at group level. e Brain BLI at three weeks (total photon count = dorsal + ventral region of interest). b-e Student’s t-test: n.s. P ≥ 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001. f Kaplan-Meier survival plot (Mantel-Cox log-rank test: *** P < 0.001). All values are given as the mean ± s.e.m. (TIFF 2165 kb)

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