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Additional file 7: of Immune microenvironment modulation unmasks therapeutic benefit of radiotherapy and checkpoint inhibition

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posted on 2019-08-14, 04:00 authored by Jared Newton, Aurelie Hanoteau, Hsuan-Chen Liu, Angelina Gaspero, Falguni Parikh, Robyn Gartrell-Corrado, Thomas Hart, Damya Laoui, Jo Ginderachter, Neeraja Dharmaraj, William Spanos, Yvonne Saenger, Simon Young, Andrew Sikora
Figure S7. Tumor immune microenvironment data time course. Flow cytometry assessment of tumor was performed at day 23, day 33, and day 37 for the CPR treatment group and major immune cell subset percentages (among CD45+ cells) are shown. (A) Percentage of CD4+ and CD8+ T cell subsets. (B) Percentage E7 tetramer+ CD8+ T cells. (C) Percentage of Tregs. (D) Percentage of major myeloid subsets. (For A-D, Tukey’s multiple comparison test; N = 2; 8–13 per group). (E) Aggregate flow cytometry scatter plots of CD8+ T cells showing E7 tetramer staining (N = 1, representative of 2; n = 4 aggregate samples per group). **p < 0.01; ***p < 0.001; ****p < 0.0001. (PDF 14226 kb)

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