Additional file 7: of Human-like NSG mouse glycoproteins sialylation pattern changes the phenotype of human lymphocytes and sensitivity to HIV-1 infection

Figure S7. Profile of human cells in NSG-cmah−/− at 9 weeks post HIV-1 infection. NSG-cmah−/− mice were infected with HIV-1ADA intraperitoneally at 5 months of age. At 9 weeks post-infection, samples were collected for FACS analyses of the peripheral blood, spleen, and bone marrow. A, Human cell profile in peripheral blood. FACS gating strategies used: human CD45/CD3/CD14; CD3/CD4/CD8; CD4/CD45RO. B, For spleen additional analysis included: human CD45/CD14/CD123/CD1c and CD45/CD19. C, Bone marrow analyses was done for CD45/CD3/CD19 and lineage negative CD3−/CD19− human CD34+ and CD33-positive cells. Individual mouse and means with SEM are shown. P values were determined with Mann-Whitney test. P ≤ 0.05 were considered significant. Reconstituted at variable levels, NSG-cmah−/− mice showed high sensitivity to HIV-1 infection with significantly decreased numbers of human T-cells (predominantly helper T-cells) and CD4+CD45RO+ memory T-cells in peripheral blood and spleen. In contrast, CD19-positive mature B-cells (spleen) and B-cell precursors (bone marrow) were significantly increased in NSG-cmah−/− mice following HIV exposure. Results for NSG mice at 9 weeks after HIV-1 infection not shown as only 3 animals were available for FACS analyses with significant variability. (PDF 951 kb)