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Additional file 7 of High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers

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posted on 2020-03-24, 04:56 authored by Sebastian Kallabis, Lena Abraham, Stefan Müller, Verena Dzialas, Clara Türk, Janica Lea Wiederstein, Theresa Bock, Hendrik Nolte, Leonardo Nogara, Bert Blaauw, Thomas Braun, Marcus Krüger
Additional file 7 : Table S2: Data for short LC-MS gradients related to Suppl. Figure 1. S2_AbsProtCopyNr: Absolute protein copy numbers per muscle fiber nucleus either calculated using the Proteomics Dynamic Range Standard (UPS2) kit or the proteomic ruler approach, related to Suppl. Figure 1A-B. S2_Identifications-1-7: Peptide and protein groups output table of different LC-MS gradients measured with the Easy nLC II-LTQ-Orbitrap Discovery system, related to Suppl. Figure 1C – E. S2_proteingroups_CAPvsnLC: Protein groups output table of single fiber ProFiT runs using the Easy nLC II - LTQ Orbitrap and capillary LC/quadrupole Orbitrap system, related to Suppl. Figure 1F. S2_peptides_CAPvsnLC: Peptide output table of single fiber ProFiT runs using the Easy nLC II - LTQ Orbitrap and capillary LC/quadrupole Orbitrap system, related to Suppl. Figure 1G. S2_CarryOver: List of log2 LFQ intensities of short gradients with six muscle fibers and blank runs to test for potential carryover, related to Suppl. Figure 1H. S2_Top5MyHCPeptides: List of MyHC peptides analyzed with ProFit with its mass, retention time and peptide scores measured on an Easy nLC II - LTQ Orbitrap or a capillary LC/quadrupole Orbitrap system, related to Suppl. Figure 1I-J

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