Additional file 7: of Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device

The modified Boyden chamber assay. A) Schematic diagram (sagittal section) of one well of the modified Boyden chamber assay (Transwell). Cells in the chemotaxis buffer are located in the upper chamber and the chemoattractant the chemotaxis buffer is added to the lower chamber. B) Schematic diagram of the membrane part of the modified Boyden chamber. The membrane separates the upper and the lower chamber. The matrix is coated on the lower side of the membrane. C) Photographs of the lower side of the membrane after the assay. Cells are stained with the staining solution accompanied with the assay kit. Magnification: 400×. (TIFF 98113 kb)