Additional file 7: of Disruption of ureide degradation affects plant growth and development during and after transition from vegetative to reproductive stages

Figure S6. Characterization of ups1 and ups2 mutants. (a) Diagram of the T-DNA insertion in AtUPS1 in the ups1 mutant. Arrows indicate PCR primers; white boxes indicate untranslated regions; black boxes indicate exons; black lines indicate introns. (b) PCR-based genotyping of the ups1 mutant using primers specific to AtUPS1 (F1 and R1) and the left border sequence of the T-DNA (SAIL LB1). (c) Diagram of the T-DNA insertion in AtUPS2 in the ups2 mutant. Arrows indicate PCR primers; white boxes indicate untranslated regions; black boxes indicate exons; black lines indicate introns. (d) PCR-based genotyping of the ups1 mutant using primers specific to AtUPS2 (F2 and R2) and the left border sequence of the T-DNA (LBa1). (e) Semi-quantitative reverse transcription-PCR for estimating AtUPS1 and AtUPS2 mRNA levels in the mutant lines. VHA-A expression was simultaneously analyzed as an internal control. (PDF 865 kb)