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Additional file 7: Figure S5. of The sialyl-glycolipid stage-specific embryonic antigen 4 marks a subpopulation of chemotherapy-resistant breast cancer cells with mesenchymal features
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posted on 2015-11-25, 05:00 authored by Andrea Aloia, Evgeniya Petrova, Stefan Tomiuk, Ute Bissels, Olivier DĂŠas, Massimo Saini, Franziska Zickgraf, Steve Wagner, Saskia Spaich, Marc SĂźtterlin, Andreas Schneeweiss, Manuel Reitberger, Silvia RĂźberg, Bernhard Gerstmayer, David Agorku, Sebastian KnĂśbel, Annalisa Terranegra, Monica Falleni, Laura Soldati, Martin Sprick, Andreas Trumpp, Jean-Gabriel Judde, Andreas Bosio, Stefano Cairo, Olaf HardtTumor-initiating capacity of the SSEA4-positive and SSEA4-negative subpopulation. (a) One hundred thousand freshly dissociated SSEA4-positive or SSEA4-negative cells were injected in two groups of eight mice each. Tumor volume was measured once per week, and the mean volume of both groups was calculated. The significance level (p value by t test) is indicated above each time point. (b) The frequency of SSEA4-expressing cells in the parental tumor model HBCx-14 and in tumors that originated from the SSEA4-positive or SSEA4-negative subpopulation was determined by flow cytometry, which indicated a regulation of SSEA4 expression back to the initial level during the growth phase in vivo. (PNG 93 kb)
