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Additional file 7: Figure S4. of miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1
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posted on 2017-03-04, 05:00 authored by Yanqing Liu, Rui Liu, Fei Yang, Rongjie Cheng, Xiaorui Chen, Shufang Cui, Yuanyuan Gu, Wu Sun, Chaoying You, Zhijian Liu, Feng Sun, Yanbo Wang, Zheng Fu, Chao Ye, Chenyu Zhang, Jing Li, Xi ChenEffect of miR-19a on TIA1 mRNA level and the efficacies of miR-19a probe and TIA1 mRNA probes. (A) Quantitative RT-PCR analysis of TIA1 mRNA levels in SW480, Caco2 and HT-29 cells transfected with control mimic, miR-19a mimic, control inhibitor or miR-19a inhibitor. (B and C) Western blot analysis of TIA1 protein levels in SW480 cells transfected with control mimic, miR-19a mimic or biotinylated miR-19a mimic. B: representative images; C: quantitative analysis. (D) Quantitative RT-PCR analysis of TIA1 and GAPDH mRNA levels in SW480 after pulling down with control probe or TIA1 mRNA probes. **P < 0.01; ***P < 0.001. (TIF 371 kb)