Additional file 7: Figure S4. of miR-19a promotes colorectal cancer proliferation and migration by targeting TIA1

Effect of miR-19a on TIA1 mRNA level and the efficacies of miR-19a probe and TIA1 mRNA probes. (A) Quantitative RT-PCR analysis of TIA1 mRNA levels in SW480, Caco2 and HT-29 cells transfected with control mimic, miR-19a mimic, control inhibitor or miR-19a inhibitor. (B and C) Western blot analysis of TIA1 protein levels in SW480 cells transfected with control mimic, miR-19a mimic or biotinylated miR-19a mimic. B: representative images; C: quantitative analysis. (D) Quantitative RT-PCR analysis of TIA1 and GAPDH mRNA levels in SW480 after pulling down with control probe or TIA1 mRNA probes. **P < 0.01; ***P < 0.001. (TIF 371 kb)