Additional file 7: Figure S13. of Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine

Immunofluorescent staining for TREM-1 and macrophage (CD14) and for colocalization of TREM-1 with CD14+ cells in articular cartilage, tendon, ligament, and suprapatellar fat. Immunofluorescent staining for TREM-1 (a, e, i, and m), CD14 (b, f, j, and n), and DAPI (c, g, k, and o) was performed, and stains were merged to examine colocalization of TREM-1 and CD14 (d, h, l, and p). Arrows show the colocalization of TREM-1 with CD14. These are the representative images of five swine each in the VDDef and VDSuff groups and three swine in the VDSupp group. (TIF 107810 kb)