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Additional file 6: of Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

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posted on 2019-01-14, 05:00 authored by Wataru Matsunaga, Hanako Shimura, Senri Shirakawa, Reika Isoda, Tsuyoshi Inukai, Takeshi Matsumura, Chikara Masuta
Figure S5. 35S promoter sequences in the viral genomes of the vector isolated from infected tissues. a-c Sequencing chromatograms of the inserts containing each of 345-, 208- and 116-bp portion (a to c, respectively). Total RNA was isolated 15 days postinoculation, and RT-PCR-amplified fragment were directly sequenced. We confirmed that the original sequences integrated into the viral vector did not change in the infected tissues. (TIFF 7535 kb)

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