Additional file 6: of Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

Figure S5. 35S promoter sequences in the viral genomes of the vector isolated from infected tissues. a-c Sequencing chromatograms of the inserts containing each of 345-, 208- and 116-bp portion (a to c, respectively). Total RNA was isolated 15 days postinoculation, and RT-PCR-amplified fragment were directly sequenced. We confirmed that the original sequences integrated into the viral vector did not change in the infected tissues. (TIFF 7535 kb)