Additional file 6: of The molecular mechanism of LncRNA34a-mediated regulation of bone metastasis in hepatocellular carcinoma

MiR-34a targets Smad4 to inhibit TGF-β1-induced target expression and migration of hepatoma cells. Figure S3. MiR-34a targets Smad4 to inhibit TGF-β1-induced target expression and migration of hepatoma cells (n = 3). Validation of transfection expression in SMMC-7721 and HCC-LM3 cells (A and B). qRT-PCR (C and D), ELISA (E), Western blot (F), wound-healing assay (G; Magnification × 40) and transwell assay (Magnification: × 100) without (H) or with (I) Matrigel and CCK-8 assay (J), were conducted in SMMC-7721 cells transfected with miR-34a kd, shSmad4, miR-34a kd plus shSmad4 or NC, and in HCC-LM3 cells transfected with miR-34a, Smad4, miR-34a plus Smad4 or NC, induced with TGF-β1. NC, negative control. *P < 0.05; **P < 0.01; ***P < 0.001. (TIF 6.18 MB)