Additional file 6: of Stability of gene expression by primary bronchial epithelial cells over increasing passage number

Figure S5. Un-normalized mRNA expression of genes associated with airway epithelial basal cells, ciliogenesis, and epithelial tight junctions by primary BECs. Un-normalized mRNA Ct values for TP63 (A.), KRT5 (B.), TUBB4A (C.), TPJ1 (D.), FOXJ1 (E.), and OCLN (F.) by BECs at P1 (n = 6 asthma donors, n = 6 healthy donors), P2 (n = 6 asthma donors, n = 6 healthy donors), P3 (n = 4 asthma donors, n = 6 healthy donors), P4 (n = 6 asthma donors, n = 6 healthy donors), and P5 (n = 6 asthma donors, n = 6 healthy donors) are presented as individual data points for each donor cell line. To compare expression of genes at P2-P5 to expression at P1, and to compare patterns of gene expression between asthmatic and healthy donors, ordinary two-way ANOVA with Dunnett’s multiple comparisons test was used for normally distributed data, and Kruskal-Wallis ANOVA with Dunn’s multiple comparisons test was used for non-normally distributed data. (EMF 224 kb)